Campylobacter fetus is Internalized by Bovine Endometrial Epithelial Cells

Hippurate hydrolysis by Campylobacter fetus.

An additional method for differentiating between Campylobacter fetus subsp. jejuni and C. fetus subsp. intestinalis is reported. Strains of C. fetus subsp. jejuni (18/20) were shown to hydrolyze hippurate in the 2-h rapid test, whereas strains of C. fetus subsp. intestinalis did not.

Establishment and characterization of immortalized bovine endometrial epithelial cells

Bovine primary uterine endometrial epithelial cells (EECs) are not ideal for long-term studies, because primary EECs lose hormone responsiveness quickly, and/or they tend to have a short life span. The aims of this study were to establish immortalized bovine EECs and to characterize these cells following long-term cultures. Immortalized bovine EECs were established by transfecting retroviral ve...

Sildenafil Effect on Nitric Oxide Secretion by Normal Human Endometrial Epithelial Cells Cultured In vitro

Effects of conjugated linoleic acids on prostaglandin secretion by bovine endometrial epithelial cells in vitro.

OBJECTIVE To determine the effects of 2 conjugated linoleic acid (CLA) isomers (cis-9, trans-11 and trans-10, cis-12) on synthesis of prostaglandin (PG) E(2) and F(2α) and expression of prostaglandin H synthase-2 (PGHS-2) of adult and fetal bovine endometrial epithelial cells in vitro. SAMPLE Primary cultures of endometrial epithelial cells obtained from 4 adult cows and 4 fetal bovine carcas...

Distinct phospholipase A2 enzymes regulate prostaglandin E2 and F2alpha production by bovine endometrial epithelial cells

BACKGROUND The rate-limiting step in prostaglandin (PG) biosynthesis is catalyzed by phospholipase A2 (PLA2) enzymes which hydrolyze arachidonic acid from membrane phospholipids. Despite their importance in uterine PG production, little is known concerning the specific PLA2 enzymes that regulate arachidonic acid liberation in the uterine endometrium. The objectives of this study were to evaluat...